Rat Fibroblast Growth Factor 8 (FGF-8) ELISA Kit from MyBioSource.com

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Rat Fibroblast Growth Factor 8 (FGF-8) ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Interleukin 26 (also known as IL-26) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat IL-26 antibody. IL-26 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat IL-26 Antibody is added and binds to IL-26 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-26 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat IL-26. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm